The Potential of Tapak Dara (<i>Catharanthus roseus</i>) Leaves Endophytic Bacteria BETD5 as Antioxidant and Anticancer Against T47D Breast Cancer Cells

BETD5 is an endophytic bacteria from Tapak Dara (Catharanthus roseus) leaves that have the potential as an antioxidant and anticancer. Therefore, this study aimed to measure antioxidant activity, characterize biochemical properties, determine anticancer activity againstT47D cells line, and identify the secondary metabolites of endophytic bacteria Tapak Dara of BETD5 by Gas Chromatography-Mass Spectrometry (GCMS). The method used was experimental and the parameters observed were antioxidant and anticancer activities, consisting of IC50 (Inhibition Concentration) and biochemical properties. Antioxidant activity was measured using various sample extract concentrations, namely 5ppm, 10ppm, 25ppm, 50ppm, and 100ppm. Biochemical tests were carried out by Triple Sugar Iron Agar (TSIA), Simmons Citrate (SC), motility, indole, catalase, and Methyl Red (MR) tests. Cytotoxicity test on cells line T47D was performed by MTT assay to evaluate anticancer activity using concentrations of 3.125, 6.25, 12.5, 25, and 50 ppm. The results showed that antioxidant activity of BETD5 was strong because IC50 value obtained was 66.27 ppm. Furthermore, biochemical tests revealed that BETD5 belonged to the genus Staphylococcus, while cytotoxicity activity obtained an IC50 value of 14.28 ppm. This indicated that endophytic bacteria extract of BETD5 had moderate cytotoxicity against T47D breast cancer cells line. The highest secondary metabolite in endophytic bacteria isolate BETD5 was cis-Ocimene (24.31%) based on GCMS analysis.