Responses of giving n-hexane Jaloh extract on broiler chicken recieved heat stress on expression of iNOS enzyme in lung tissue, serum level of glucose and calcium

Giving extract n-hexane of jaloh bark (EHJ) lessen the impact of heat stress in broiler chicken. The studies of activity of EHJ in lessening heat stress impact at broiler chicken have not been conducted yet. An experiment was conducted to study the effect of extract n-hexane of jaloh bark (EHJ) administration on body temperature, level of calcium and glucose in serum, inducible nitric oxide synthase (iNOS) in lung tissue, and behavior of broilers exposed to heat stress at temperature 33 +/- 1(0)C. In this study 18 broiler chicks aged 20 days were divided into 3 groups. The first group was broilers without heat stress nor EHJ (control). The second group was broilers given heat stress without EHJ (CP). The third group was treated heat stress and EHJ at dose of 10 mg/kg BW (CP+EHJ). The EHJ was given 1 hour before cage temperature was raised. Broilers in group CP and CP+EHJ were divided into 3 periods of slaughtering i.e. 2 and 4 hours after given heat stress and 2 hours after heat stress termination. Each of slaughtering period consisted of 3 broilers as replication. Result of this research indicated that CP+EHJ lessen stress in the form of restlessness and increased expression of iNOS in the lung tissue. It was assumed that one of mechanism of EHJ in lessening stress on broiler related to forming of iNOS enzyme in the lung tissue.