Characterization and Identification of Thermostable Alkaline Lipase Producing Bacteria from Hot Spring around West Java

Thermophilic lipase producing microorganisms were isolated from a few hot springs around West Java, Indonesia Eight isolates showed lipolytic activity on agar plate media containing olive oil and rhodamine B Three best lipase producers, namely DMS3, KHA_P12, and KHA_T25, were further characterized and showed maximum specific activity at a mid exponential of growth phase Identification of the isolates were performed through ribotyping method Chromosomal DNA from these isolates were extracted and the 16S rRNA gene fragments were amplified, resulting fragment length at around 1 5 kb on agarose gel Further analysis of the 16S rRNA gene sequences using homological and phylogenetic methods showed that the DMS3, KHA_P12, and KHA_T25 were unique strains, however close to Geobacillus kaustophilus, Therm us aquaticus, and Geobacillus stearothermophilus respectively